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Enzo Life Sciences欣博盛生物:NUCLEAR-ID®系列染料

更新時間:2023-07-27   點擊次數(shù):370次

Enzo Life Sciences公司的NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)NUCLEAR-ID ® Red DNA Stain是細胞滲透性染料,適用于活細胞的細胞核染色。這系列染料為通過流式細胞術研究細胞周期進程的誘導和抑制提供了一種簡便方法。該試劑在活細胞研究中的潛在應用包括確定細胞DNA含量和細胞周期分布、檢測生長模式的變化、監(jiān)測細胞凋亡以及評估腫瘤細胞行為和抑制基因機制。

 

NUCLEAR-ID® Red DNA stain

 

產(chǎn)品特點

● 遠紅色熒光特異性DNA染料

● 穩(wěn)定且純度高

● 可分析活細胞、可滲透細胞和固定細胞

● 無光漂白作用

● 無需RNase處理

● GFPFITC兼容

● 不需要紫外線激光源激發(fā)

● 經(jīng)驗證適用于各種細胞密度

● 快速且易于使

 

實例分析

37°C下用終濃度為4000x2000xNUCLEAR-ID ® Red DNA Stain同等µM濃度的競爭品牌染料對細胞進行染色,并在染色后輕輕洗滌。15分鐘24小時細胞成像,結果顯示NUCLEAR-ID ® Red DNA Stain在更低的濃度就能顯現(xiàn)dsDNA


ENZO熱銷產(chǎn)品——NUCLEAR-ID®系列染料

 

產(chǎn)品詳情:

產(chǎn)品名稱

NUCLEAR-ID® Red DNA stain

產(chǎn)品貨號

ENZ-52406

產(chǎn)品規(guī)格

200µl

最大激發(fā)波長

566nm

最大發(fā)射波長

650nm

純度

≥93%HPLC

質量控制

1.Absorption peak of NUCLEAR-ID® Red dye: λmax = 566 ± 4 nm

2.% purity of NUCLEAR-ID® Red dye by HPLC: ≥93%

應用

Flow Cytometry, Fluorescent detection

短期儲存

-20°C

長期儲存

-80°C

產(chǎn)品說明

The NUCLEAR-ID® Red DNA Stain is a member of the CELLESTIAL® product line, reagents and assay 

kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis 

applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such 

as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

 

 

部分產(chǎn)品文獻引用

1. CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils: M.I.P. Ramos, et al.; J. Leukoc. Biol. 111, 367 (2022)

2. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers: A.P. Sawaya, et al.; EMBO Rep. 23, e54558 (2022)

3. Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample: H. Jeon, et al.; Sci. Rep. 12, 4212 (2022)

4. DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells: B. Chen, et al.; Int. J. Mol. Sci. 22, 4011 (2021)

5. Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells: A. Recasens, et al.; Cell Death Discov. 7, 81 (2021)

6. Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells; Journal of Innate Immunity: S. Surabhi, et al.; J. Innate Immun. 10, 1159 (2021)

7. Licochalcone A inhibits hypoxia-inducible factor-1α accumulation by suppressing mitochondrial respiration in hypoxic cancer cells: M.K. Park, et al.; Biomed. Pharmacother. 133, 111082 (2021)

 

NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)

 

產(chǎn)品特點

● 藍色熒光特異性DNA染料

● 穩(wěn)定且純度高

● 可分析活細胞、可滲透細胞和固定細胞

● 無光漂白作用

● 無需RNase處理

● GFP和紅色染料兼容

● 經(jīng)驗證適用于各種細胞密度

● 快速易用

 

產(chǎn)品詳情:

產(chǎn)品名稱

NUCLEAR-ID® Blue DNA stain (GFP-CERTIFIED®)

產(chǎn)品貨號

ENZ-CHM103-0200

產(chǎn)品規(guī)格

200µl

最大激發(fā)波長

350nm

最大發(fā)射波長

461nm

純度

≥95% (HPLC)

外觀

Frozen liquid

應用

Flow Cytometry, Fluorescent detection

短期儲存

-20°C, Protect from light. Avoid freeze/thaw cycles

長期儲存

-80°C, Protect from light. Avoid freeze/thaw cycles

 


 ENZO熱銷產(chǎn)品——NUCLEAR-ID®系列染料

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